Cell cycle-dependent regulation of phosphorylation of the human retinoblastoma gene product.

@article{Mihara1989CellCR,
  title={Cell cycle-dependent regulation of phosphorylation of the human retinoblastoma gene product.},
  author={Kouichiro Mihara and Xiao-zhe Cao and Andrew Ying-Ming Yen and S. R. Chandler and Barbara A Driscoll and A. Linn Murphree and A T'ang and Y. T. Fung},
  journal={Science},
  year={1989},
  volume={246 4935},
  pages={1300-3}
}
The human retinoblastoma gene (RB1) encodes a protein (Rb) of 105 kilodaltons that can be phosphorylated. Analysis of Rb metabolism has shown that the protein has a half-life of more than 10 hours and is synthesized at all phases of the cell cycle. Newly synthesized Rb is not extensively phosphorylated (it is "underphosphorylated") in cells in the G0 and G1 phases but is phosphorylated at multiple sites at the G1/S boundary and in S phase. HL-60 cells that were induced to terminally… CONTINUE READING