Cell cycle-dependent localization of monoclonal antibodies raised against isolated Dictyostelium centrosomes.

@article{Grf1999CellCL,
  title={Cell cycle-dependent localization of monoclonal antibodies raised against isolated Dictyostelium centrosomes.},
  author={Ralph O Gr{\"a}f and Christine Daunderer and Manfred Schliwa},
  journal={Biology of the cell},
  year={1999},
  volume={91 6},
  pages={471-7}
}
The ultrastructure of the Dictyostelium centrosome is markedly different from that of the well known yeast spindle pole body and vertebrate centriole-containing centrosome. It consists of a box-shaped, layered core structure surrounded by a corona with dense nodules embedded in an amorphous matrix. For further structural and biochemical analyses of this type of centrosome we used highly enriched isolated Dictyostelium centrosomes as an antigen to raise 14 new centrosomal monoclonal antibodies… CONTINUE READING
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