Cell culture assay for transient replication of human and animal papillomaviruses.

Abstract

This unit contains protocols for evaluation of replication functionality of papillomavirus genomes or subgenomic fragments. Replication is measured after transient cotransfection of the genome (or subgenomic fragment) with expression vectors encoding the viral E1 and E2 proteins. Input DNA is methylated at the adenine of GATC sequences by propagation in E… (More)
DOI: 10.1002/9780471729259.mc14b01s00

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Cite this paper

@article{Wilson2005CellCA, title={Cell culture assay for transient replication of human and animal papillomaviruses.}, author={Van G. Wilson}, journal={Current protocols in microbiology}, year={2005}, volume={Chapter 14}, pages={Unit14B.1} }