Cell culture and infection system for hepatitis C virus

  title={Cell culture and infection system for hepatitis C virus},
  author={Takanobu Kato and Tomoko Date and Asako Murayama and Kenichi Morikawa and Daisuke Akazawa and Takaji Wakita},
  journal={Nature Protocols},
Hepatitis C virus (HCV) infection causes chronic liver disease and is a worldwide health problem. Despite ever-increasing demand for knowledge on viral replication and pathogenesis, detailed analysis has been hampered by a lack of efficient viral culture systems. We isolated HCV genotype 2a strain JFH-1 from a patient with fulminant hepatitis. This strain replicates efficiently in Huh7 cells. Efficient replication and secretion of recombinant viral particles can be obtained in cell culture by… CONTINUE READING


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