Cell culture: building a better matrix

  title={Cell culture: building a better matrix},
  author={Nathan S. Blow},
  journal={Nature Methods},
  • N. Blow
  • Published 1 August 2009
  • Biology, Engineering
  • Nature Methods
With the realization that cells interact extensively with their surrounding microenvironments during growth and development, the challenge for researchers has become designing three-dimensional culture systems that more closely mimic those relationships. 
Magnetic assembly of 3D cell clusters: visualizing the formation of an engineered tissue
Contactless magnetic assembly of cells into 3D clusters has been evaluated by visualizing the evolution of cell clusters assembled by magnetic forces, to examine their resemblance to in vivo tissues.
Matrigel: A complex protein mixture required for optimal growth of cell culture
The ability to identify the low mass and abundance components of Matrigel illustrates the utility of this method for the analysis of the extracellular matrix, as well as the complexity of the matrix itself.
Cultivation of human neural progenitor cells in a 3-dimensional self-assembling peptide hydrogel.
A method to release cells from the 3D-scaffolds for the later analysis e.g. by flow cytometry, which speeds up the analysis and more over, the obtained data rest upon a wider base, improving the reliability of the data.
ECM analog technology: a simple tool for exploring cell-ECM dynamics.
Some of the tools for cell culture in 3D, made available through novel cell-interactive ECM analog technology, are discussed, with the potential to improve the understanding about cellular behavior, both in normal and abnormal surroundings.
Diverse 3D Microarchitectures Made by Capillary Forming of Carbon Nanotubes
A new technology called capillary forming enables transformation of vertically aligned nanoscale filaments into complex three-dimensional microarchitectures. We demonstrate capillary forming of
Expansion of human pluripotent stem cells
Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms
A hydrogel with dynamic mechanics and a biochemistry based on an addition-fragmentation chain transfer (AFCT) reaction was constructed, which has great potential as a 3D cell culture platform for tissue engineering applications.
Differentiation of Human Neural Progenitor Cells in Functionalized Hydrogel Matrices
A combination of elevated neuronal differentiation and a protective effect of the modified matrices underlies the increased proportion of neuronal cells in PuraMatrix modified by the addition of short peptide sequences.
Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting
3D capillary beds in an in vitro microfluidic platform that is comprised of a biocompatible collagen I gel supported by a mechanical framework of alginate beads are reported, offering a modular method to encapsulate and co-culture cells that either promote angiogenesis or require perfusion for cell viability in engineered tissue constructs.


Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering
Although modern synthetic biomaterials represent oversimplified mimics of natural ECMs lacking the essential natural temporal and spatial complexity, a growing symbiosis of materials engineering and cell biology may ultimately result in synthetic materials that contain the necessary signals to recapitulate developmental processes in tissue- and organ-specific differentiation and morphogenesis.
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
We report a strategy to create photodegradable poly(ethylene glycol)–based hydrogels through rapid polymerization of cytocompatible macromers for remote manipulation of gel properties in situ.
Biomechanical forces promote embryonic haematopoiesis
A critical role for biomechanical forces in haematopoietic development is revealed in mouse embryos using mouse embryonic stem cells differentiated in vitro and that abrogation of nitric oxide, a mediator of shear-stress-induced signalling, compromises haem atopOietic potential in vivo and in vivo is revealed.
Supplemental Data Matrix Elasticity Directs Stem Cell Lineage Specification
Raw data was normalized by total actin levels and ranges from 0 (no expression) to 1 (maximal expression).
Hematopoietic stem cell development is dependent on blood
  • flow. Cell 137,
  • 2009
lineage specification
  • 2006
Matrix elasticity direct stem
  • cell