Cell culture: building a better matrix

@article{Blow2009CellCB,
  title={Cell culture: building a better matrix},
  author={Nathan S. Blow},
  journal={Nature Methods},
  year={2009},
  volume={6},
  pages={619-622}
}
  • N. Blow
  • Published 1 August 2009
  • Biology, Engineering
  • Nature Methods
With the realization that cells interact extensively with their surrounding microenvironments during growth and development, the challenge for researchers has become designing three-dimensional culture systems that more closely mimic those relationships. 
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30 Citations
Background Citations
9
Results Citations
1

30 Citations

Citation Type

Citation Type

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Contactless magnetic assembly of cells into 3D clusters has been evaluated by visualizing the evolution of cell clusters assembled by magnetic forces, to examine their resemblance to in vivo tissues.
Matrigel: A complex protein mixture required for optimal growth of cell culture
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The ability to identify the low mass and abundance components of Matrigel illustrates the utility of this method for the analysis of the extracellular matrix, as well as the complexity of the matrix itself.
Cultivation of human neural progenitor cells in a 3-dimensional self-assembling peptide hydrogel.
TLDR
A method to release cells from the 3D-scaffolds for the later analysis e.g. by flow cytometry, which speeds up the analysis and more over, the obtained data rest upon a wider base, improving the reliability of the data.
ECM analog technology: a simple tool for exploring cell-ECM dynamics.
TLDR
Some of the tools for cell culture in 3D, made available through novel cell-interactive ECM analog technology, are discussed, with the potential to improve the understanding about cellular behavior, both in normal and abnormal surroundings.
Diverse 3D Microarchitectures Made by Capillary Forming of Carbon Nanotubes
A new technology called capillary forming enables transformation of vertically aligned nanoscale filaments into complex three-dimensional microarchitectures. We demonstrate capillary forming of…
Expansion of human pluripotent stem cells
Hydrogels with Dynamically Controllable Mechanics and Biochemistry for 3D Cell Culture Platforms
TLDR
A hydrogel with dynamic mechanics and a biochemistry based on an addition-fragmentation chain transfer (AFCT) reaction was constructed, which has great potential as a 3D cell culture platform for tissue engineering applications.
Differentiation of Human Neural Progenitor Cells in Functionalized Hydrogel Matrices
TLDR
A combination of elevated neuronal differentiation and a protective effect of the modified matrices underlies the increased proportion of neuronal cells in PuraMatrix modified by the addition of short peptide sequences.
Engineering of In Vitro 3D Capillary Beds by Self-Directed Angiogenic Sprouting
TLDR
3D capillary beds in an in vitro microfluidic platform that is comprised of a biocompatible collagen I gel supported by a mechanical framework of alginate beads are reported, offering a modular method to encapsulate and co-culture cells that either promote angiogenesis or require perfusion for cell viability in engineered tissue constructs.
Replication of the 3D architecture of tissues
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