Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell

  title={Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell},
  author={Margaret R. Lindsay and Richard Webb and Marc Strous and Mike S. M. Jetten and Margaret K. Butler and Rebecca J. Forde and John A Fuerst},
  journal={Archives of Microbiology},
Abstract. The organisation of cells of the planctomycete species Pirellula marina, Isosphaera pallida, Gemmata obscuriglobus, Planctomyces maris and "Candidatus Brocadia anammoxidans" was investigated based on ultrastructure derived from thin-sections of cryosubstituted cells, freeze-fracture replicas, and in the case of Gemmata obscuriglobus and Pirellula marina, computer-aided 3-D reconstructions from serial sections of cryosubstituted cells. All planctomycete cells display a peripheral… 

Membrane-bounded nucleoids and pirellulosomes of planctomycetes

Compartmentalized cells of members of the distinctive phylum Planctomycetes, especially those of Gemmata strains, form an exception to the prokaryote cell plan and have significant implications for models for origins of the eukaryote nucleus.

The cell cycle of the planctomycete Gemmata obscuriglobus with respect to cell compartmentalization

The division cycle of the nucleated planctomycete G. obscuriglobus appears to be a complex process in which chromosomal DNA is transported to the daughter cell bud after initial formation of the bud, and this can be performed repeatedly by a single mother cell.

Unique Organelles in Planctomycetes

The nature of these planctomycetes compartments in relation to function and their relationship to the endomembranes defining them are examined, and the implications of the remarkable compartment-confined process of protein uptake in Gemmata are discussed.

Intracellular compartmentation in planctomycetes.

  • J. Fuerst
  • Biology, Engineering
    Annual review of microbiology
  • 2005
The phylum Planctomycetes of the domain Bacteria consists of budding, peptidoglycan-less organisms important for understanding the origins of complex cell organization, in which the nucleoid is enveloped in two membranes to form a nuclear body analogous to the structure of a eukaryotic nucleus.

The order Planctomycetales, including the genera Planctomyces, Pirellula, Gemmata and Isosphaera and the Candidatus genera Brocadia, Kuenenia and Scalindua

Through the application of molecular microbial ecology techniques over the last 10 years, it has become apparent that planctomycetes are ubiquitous in a wide range of terrestrial and aquatic environments; the physiological diversity underlying this geographic ubiquity has not yet been fully explored.

Genomic Analysis Indicates the Presence of an Asymmetric Bilayer Outer Membrane in Planctomycetes and Verrucomicrobia

The functional implications of the presence of an outer membrane for the Planctomycetes cell plan and compartmentalization are discussed and a revised model including anouter membrane is proposed.

Nested Bacterial Boxes: Nuclear and Other Intracellular Compartments in Planctomycetes

The nature of these planctomycetes compartments in relation to function and their relationship to the endomembranes defining them are examined, and the implications of the remarkable compartment-confined process of protein uptake in Gemmata are discussed.

Beyond the bacterium: planctomycetes challenge our concepts of microbial structure and function

The recent discovery of both an endocytosis-like ability and proteins homologous to eukaryotic clathrin in a planctomycete marks this phylum as one to watch for future research on the origin and evolution of the eUKaryotic cell.

Cell Compartmentalization and Endocytosis in Planctomycetes: Structure and Function in Complex Bacteria

Planctomycetes are unique among the domain Bacteria in possessing cells with a complex plan defined by internal membranes forming separated compartments within the cell. They also possess other

A New Addition to the Cell Plan of Anammox Bacteria: “Candidatus Kuenenia stuttgartiensis” Has a Protein Surface Layer as the Outermost Layer of the Cell

The outermost layer of the anammox cell is investigated and identified a proteinaceous surface layer (S-layer) (a crystalline array of protein subunits) as the outermost component of the cell envelope of theAnammox bacterium "Candidatus Kuenenia stuttgartiensis" and is the first description of an S-layer in the phylum of the Planctomycetes.



Pirellulosomes: a new type of membrane-bounded cell compartment in planctomycete bacteria of the genus Pirellula.

Immuno- and cytochemical-labelling of P. marina demonstrated that DNA is located exclusively within the pirellulosome; cell RNA is concentrated in the pirespecies, with some RNA also located in the polar cap region.

Defensive extrusive ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia.

The prokaryotic vs. eukaryotic nature of epixenosomes was resolved by comparative sequence analysis of amplified small subunit rRNA genes and in situ hybridization with fluorescently labeled rRNA-targeted polynucleotide probes.

The planctomycetes: emerging models for microbial ecology, evolution and cell biology.

The planctomycetes are an unusual but widely distributed group of budding bacteria which are proving to be of increasing relevance to at least three major areas of research in microbiology: microbial

Membrane-bounded nucleoid in the eubacterium Gemmata obscuriglobus.

  • J. FuerstR. Webb
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1991
The occurrence of a membrane-bounded nucleoid in a eubacterial prokaryote is a significant exception to the evidence supporting the proKaryote/eukaryote dichotomous classification of cell structure.

Nucleoids and coated vesicles of “Epulopiscium” spp.

Parts of the anatomy of two “Epulopiscium” morphotypes, unusually large bacteria that are not yet cultured and that reproduce by the internal generation of two or more vegetative daughter cells, are described.

Membrane‐bounded nucleoids in microbial symbionts of marine sponges

In thin sections of resin-embedded samples of glutaraldehyde- and osmium tetroxide-fixed tissue from five genera of marine sponges, cells of a bacteria-like symbiont microorganism which exhibit a membrane-bounded nuclear region encompassing the fibrillar nucleoid have been observed within the sponge mesohyl.

Molecular genetic evidence for early evolutionary origin of budding peptidoglycan-less eubacteria

Comparison of data obtained from three budding, peptidoglycan-less strains with and without stalks revealed that the strains investigated are not members of the archaebacterial kingdom but represent an ancient line of descent of the eubacterial kingdom.

Membrane-bounded nuclear bodies in a diverse range of symbionts of Great Barrier Reef sponges

Thin sections of chemically fixed tissue of several sponge species collected from Heron Island, Great Barrier Reef, were examined to investigate the cell organisation of bacteria-like microbial symbionts present, finding a variety of different symbiont morphotypes found to possess a membrane-bounded nucleoid, a feature not expected in prokaryotes.

Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon

Bacteria phenotypically resembling members of the phylogenetically distinct planctomycete group of the domain Bacteria were isolated from postlarvae of the giant tiger prawn, Penaeus monodon, and demonstrated that the prawn isolates were members of that group.

16S ribosomal RNA‐ and cell wall analysis of Gemmata obscuriglobus, a new member of the order Planctomycetales

Gemmata obscuriglobus UQM2246 was investigated by the 16S ribosomal RNA cataloguing approach in order to determine its phylogenetic position, displaying a remote and equidistant relationship to representatives of the genera Planctomyces and Pirella.