Cell-cell interactions during the formation and reactivation of “nonculturable” mycobacteria

  title={Cell-cell interactions during the formation and reactivation of “nonculturable” mycobacteria},
  author={E. Salina and G. Vostroknutova and M. Shleeva and A. Kaprelyants},
To date, the possible existence of “nonculturable” (NC) but potentially viable forms has been shown for some bacteria. NC mycobacteria have attracted particular interest due to the assumption that the latent form of tuberculosis is associated with the conversion of its causative agent, Mycobacterium tuberculosis, into the NC state. A number of approaches have been developed to obtain NC forms of mycobacteria, but the mechanisms of transition into or from this state have been insufficiently… Expand
Differentially Expressed Proteins in Response to Resuscitation of Non- Culturable Cells of Mycobacterium tuberculosis H37Rv: Potential New Drug Targets
The differential expression of 14 proteins in NC and resuscitation phase was also regulated at transcriptional level as revealed by real time RT-PCR analysis and could thus become potential drug targets. Expand
Structural Changes and Cellular Localization of Resuscitation-Promoting Factor in Environmental Isolates of Micrococcus luteus
A number of environmental isolates of M. luteus show enhanced resistance to lysozyme as compared with the laboratory strains and this correlates with increased peptidoglycan acetylation, and a possible role for the Rpf linker in environmental adaptation is proposed. Expand
Finding of the Low Molecular Weight Inhibitors of Resuscitation Promoting Factor Enzymatic and Resuscitation Activity
A novel class of 2-nitrophenylthiocyanates (NPT) compounds are described that inhibit muralytic activity of Rpfs with IC50 1–7 µg/ml, the first example of low molecular weight compounds that inhibit the enzymatic and biological activities of R pf proteins. Expand
Expression, purification, and characterization of soluble RpfD with high bioactivity as a recombinant protein in Mycobacterium vaccae.
The results indicate that the sRpfD from M. vaccae could more efficiently stimulate the resuscitation of M. tuberculosis H37Ra than the refolded recombinant Rp fD from Escherichia coli DH5alpha, and that the rabbit anti- sR pfD serum could completely inhibit this resuscitation-promoting effect caused by these two kinds of recombinant rpf D. Expand
Comparative expression analysis of rpf-like genes of Mycobacterium tuberculosis H37Rv under different physiological stress and growth conditions.
Mycobacterium tuberculosis H37Rv possesses five resuscitation-promoting factors, RpfA-E, which are required for the resuscitation of dormancy in mycobacteria induced by prolonged incubation of theExpand
The Incidence and Clinical Implication of Sputum with Positive Acid-Fast Bacilli Smear But Negative in Mycobacterial Culture in a Tertiary Referral Hospital in South Korea
Clinicians should consider the possibility of false positive AFB smear, as most sputum with a positive AFB smear but negative culture could be classified as a laboratory failure. Expand


A bacterial cytokine.
A proteinaceous autocrine or paracrine bacterial growth factor or cytokine, which promotes the resuscitation and growth of dormant, nongrowing cells of the same organism, is described. Expand
Formation and resuscitation of "non-culturable" cells of Rhodococcus rhodochrous and Mycobacterium tuberculosis in prolonged stationary phase.
The observed activity of culture supernatants and Rpf with "non-culturable" bacterial suspensions invites the speculation that one, or more, of the cognate Mycobacterium tuberculosis RpF-like molecule(s) could be involved in mechanisms of latency and reactivation of tuberculosis in vivo. Expand
Viable but non-culturable and dormant bacteria: time to resolve an oxymoron and a misnomer?
  • M. Barer
  • Biology, Medicine
  • Journal of medical microbiology
  • 1997
The proposal that several pathogens which are readily culturable, including Yibrio cholerae, Campylobacter jejuni and Helicobacter pylori, may enter a state in which they retain viability but fail to grow in conventional culture strikes at the core of the equation, viability = culturability. Expand
A family of autocrine growth factors in Mycobacterium tuberculosis
It is shown here that the five cognate proteins from M. tuberculosis have very similar characteristics and properties to those of Rpf, and may provide novel opportunities for preventing and controlling mycobacterial infections. Expand
Nonreplicating persistence of mycobacterium tuberculosis.
In vitro models reinforce this conclusion that hypoxia is a major factor in inducing nonreplicating persistence (NRP) of tubercle bacilli and provide insights into mechanisms that make NRP possible. Expand
An in vitro model for sequential study of shiftdown of Mycobacterium tuberculosis through two stages of nonreplicating persistence
It is suggested that the ability to shift down into one or both of the two nonreplicating stages, corresponding to microaerophilic and anaerobic persistence, is responsible for the ability of tubercle bacilli to lie dormant in the host for long periods of time, with the capacity to revive and activate disease at a later time. Expand
Adoption of the transiently non-culturable state--a bacterial survival strategy?
The idea that cells can adopt physiological states in which they appear to be (transiently) non-culturable is developed, and the morphological, physiological and genetic evidence underpinning such behaviour and its adaptive significance is summarised. Expand
Microbial persistence. I. The capacity of tubercle bacilli to survive sterilization in mouse tissues.
The persisting tubercle bacilli are more correctly designated as being in a "sterile state" than one of true latency, as this phenomenon is a specific phenomenon requiring the participation of both the nicotinamide derivative, pyrazinamide, and isoniazid. Expand
Formation of 'non-culturable' cells of Mycobacterium smegmatis in stationary phase in response to growth under suboptimal conditions and their Rpf-mediated resuscitation.
In contrast to wild-type, the NC cells of purF and devR mutants obtained under oxygen-limited conditions resuscitate spontaneously, presumably because the heterogeneous population contains some residual viable cells that continue to make Rpf-like proteins. Expand
Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases
The data indicate that Rpf is a peptidoglycan-hydrolyzing enzyme, and strongly suggest that this specific activity is responsible for its growth promotion and resuscitation activity. Expand