Cell-based high-throughput screening assay system for monitoring G protein-coupled receptor activation using beta-galactosidase enzyme complementation technology.

@article{Yan2002CellbasedHS,
  title={Cell-based high-throughput screening assay system for monitoring G protein-coupled receptor activation using beta-galactosidase enzyme complementation technology.},
  author={Yu-Xin Yan and Deborah M Boldt-Houle and Bonnie P Tillotson and Melissa A. Gee and Brian J D'Eon and Xiao-Jia Chang and Corinne E M Olesen and Michelle Palmer},
  journal={Journal of biomolecular screening},
  year={2002},
  volume={7 5},
  pages={451-9}
}
A novel cell-based functional assay to directly monitor G protein-coupled receptor (GPCR) activation in a high-throughput format, based on a common GPCR regulation mechanism, the interaction between beta-arrestin and ligand-activated GPCR, is described. A protein-protein interaction technology, the InteraX trade mark system, uses a pair of inactive beta-galactosidase (beta-gal) deletion mutants as fusion partners to the protein targets of interest. To monitor GPCR activation, stable cell lines… CONTINUE READING

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