Cell-based bioreporter assay coupled to HPLC micro-fractionation in the evaluation of antimicrobial properties of the basidiomycete fungus Pycnoporus cinnabarinus.

@article{Jrvinen2016CellbasedBA,
  title={Cell-based bioreporter assay coupled to HPLC micro-fractionation in the evaluation of antimicrobial properties of the basidiomycete fungus Pycnoporus cinnabarinus.},
  author={P{\"a}ivi P J{\"a}rvinen and Susanna Nybond and Laurence Marcourt and Emerson Ferreira Queiroz and Jean-Luc Wolfender and Aila Mett{\"a}l{\"a} and Matti T. Karp and Heikki Vuorela and Pia Maarit Vuorela and Annele I. Hatakka and P{\"a}ivi Tammela},
  journal={Pharmaceutical biology},
  year={2016},
  volume={54 6},
  pages={
          1108-15
        }
}
CONTEXT Identification of bioactive components from complex natural product extracts can be a tedious process that aggravates the use of natural products in drug discovery campaigns. OBJECTIVE This study presents a new approach for screening antimicrobial potential of natural product extracts by employing a bioreporter assay amenable to HPLC-based activity profiling. MATERIALS AND METHODS A library of 116 crude extracts was prepared from fungal culture filtrates by liquid-liquid extraction… CONTINUE READING

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