Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.

@article{Mateos1992CellassociatedPA,
  title={Cell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.},
  author={Pedro Francisco Mateos and Jos{\'e} Ignacio Jim{\'e}nez-Zurdo and J Chen and Andrea Squartini and Sheridan Kidd Haack and Eustoquio Mart{\'i}nez-Molina and D. H. Hubbell and Frank B. Dazzo},
  journal={Applied and environmental microbiology},
  year={1992},
  volume={58 6},
  pages={1816-22}
}
The involvement of Rhizobium enzymes that degrade plant cell wall polymers has long been an unresolved question about the infection process in root nodule symbiosis. Here we report the production of enzymes from Rhizobium leguminosarum bv. trifolii that degrade carboxymethyl cellulose and polypectate model substrates with sensitive methods that reliably detect the enzyme activities: a double-layer plate assay, quantitation of reducing sugars with a bicinchoninate reagent, and activity gel… CONTINUE READING