Superhydrophobic surfaces have been studied extensively for their valuable properties. We fabricated a superhydrophobic surface using a replica anodic aluminum oxide (AAO) template, and grew 3T3-Swiss albino cells on it and on an aluminum, polytetrafluoroethylene (PTFE) surface and a fabricated surface with a superhydrophobic property. As a result by comparison with an aluminum surface and PTFE surface, which exhibited fouling within 3 days, a superhydrophobic surface exhibited high resistance to fouling. It was very difficult for cells to remain and grow on the superhydropbobic surface. There appears to be a correlation between the nature of the superhydrophobic property and the adhesion of the cells. There appears to be a correlation between the nature of the nanoscale roughness in the creation of a superhydrophobic surface.