Cell Type–dependent Requirement for PIP Box–regulated Cdt1 Destruction During S Phase

@inproceedings{Lee2010CellTR,
  title={Cell Type–dependent Requirement for PIP Box–regulated Cdt1 Destruction During S Phase},
  author={Hyun O. Lee and Sima J. Zacharek and Yue Xiong and Robert J. Duronio},
  booktitle={Molecular biology of the cell},
  year={2010}
}
DNA synthesis-coupled proteolysis of the prereplicative complex component Cdt1 by the CRL4(Cdt2) E3 ubiquitin ligase is thought to help prevent rereplication of the genome during S phase. To directly test whether CRL4(Cdt2)-triggered destruction of Cdt1 is required for normal cell cycle progression in vivo, we expressed a mutant version of Drosophila Cdt1 (Dup), which lacks the PCNA-binding PIP box (Dup(ΔPIP)) and which cannot be regulated by CRL4(Cdt2). Dup(ΔPIP) is inappropriately stabilized… CONTINUE READING

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Highly Influential
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