Cell Fusion-Monoclonal Antibody Immunoadsorbent Cascades 3835

  title={Cell Fusion-Monoclonal Antibody Immunoadsorbent Cascades 3835},
  author={Timothy A Springer},
To simplify the construction of monoclonal antibody (MAb) libraries to complex biological systems, the use of a cascade procedure has been investigated. The procedure continually restricts MAb production to those antigens previously so unidentified. Murine macrophage membranes were detergent-solubilized and purified by Lens culinaris lectin affinity chromatography. Prior to immunization of rats, the previously recognized heat-stable antigen and common leukocyte antigen were removed with MAb… CONTINUE READING