Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast

  title={Cell-Cycle Analyses Using Thymidine Analogues in Fission Yeast},
  author={Silje Anda and Erik Boye and Be{\'a}ta Grallert},
  booktitle={PloS one},
Thymidine analogues are powerful tools when studying DNA synthesis including DNA replication, repair and recombination. However, these analogues have been reported to have severe effects on cell-cycle progression and growth, the very processes being investigated in most of these studies. Here, we have analyzed the effects of 5-ethynyl-2'-deoxyuridine (EdU) and 5-Chloro-2'-deoxyuridine (CldU) using fission yeast cells and optimized the labelling procedure. We find that both analogues affect the… CONTINUE READING


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