Cell Culture Evaluation of the Semliki Forest Virus Expression System As a Novel Approach for Antigen Delivery and Expression in Fish

@article{Phenix2000CellCE,
  title={Cell Culture Evaluation of the Semliki Forest Virus Expression System As a Novel Approach for Antigen Delivery and Expression in Fish},
  author={K. Phenix and B. McKenna and R. Fitzpatrick and L. Vaughan and G. Atkins and P. Liljestrom and D. Todd},
  journal={Marine Biotechnology},
  year={2000},
  volume={2},
  pages={27-37}
}
Abstract: Heterologous gene expression by Semliki Forest virus (SFV) expression vectors was investigated in fish cell culture. Experiments performed using an infectious strain of SFV, replication-defective SFV particles, and recombinant SFV RNA constructs encoding the Escherchia coli LacZ or firefly luciferase reporter genes indicated that levels of SFV-mediated expression in fish cells were dependent on cell type and temperature. Maximal expression levels were observed in the two salmonid… Expand
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