Cefotaxime-Hydrolysing Beta Lactamases in Morganella morganii


 The frequency of enterobacterial isolates with high resistance to expanded-spectrum β-lactam antibiotics (mainly cefotaxime or ceftriaxone) has increased notoriously in Argentina, mainly because of the spread of extended-spectrum β-lactamases. The aim of this work was the study of extended-spectrum β-lactamases in several Morganella morganii isolates with unusually high resistance to ceftriaxone. These strains produced at least two β-lactamases, of apparent pIs of 5.4 and 8.2, molecular weight 23 000, well inhibited by clavulanate, compatible with a broad-spectrum β-lactamase – perhaps TEM-1 – and an extended-spectrum β-lactamase, respectively. The extended-spectrum β-lactamase was identified as a CTX-M-type β-lactamase – probably CTX-M-2 – by polymerase chain reaction, restriction profile analysis and DNA-DNA hybridisation. The remaining isolates studied produced either the broad-spectrum β-lactamase plus the ubiquitous AmpC β-lactamase (13 strains), or the AmpC β-lactamase only (10 strains).

DOI: 10.1007/s100960050391

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@article{Power1999CefotaximeHydrolysingBL, title={Cefotaxime-Hydrolysing Beta Lactamases in Morganella morganii}, author={Patricia D. Power and Matteo Radice and Claudia Barberis and Carmen De Mier and Marta Eugenia Mollerach and M. Maltagliatti and Carlos Alberto Vay and Andrew Famiglietti and Gabriel Osvaldo Gutkind}, journal={European Journal of Clinical Microbiology and Infectious Diseases}, year={1999}, volume={18}, pages={743-747} }