Cdc2-cyclin B phosphorylates p70 S6 kinase on Ser411 at mitosis.

  title={Cdc2-cyclin B phosphorylates p70 S6 kinase on Ser411 at mitosis.},
  author={Philip J. Papst and Hiroyuki Sugiyama and Masahiro Nagasawa and Jos{\'e} J Lucas and James L. Maller and Nobuo Terada},
  journal={The Journal of biological chemistry},
  volume={273 24},
The carboxyl terminus of p70 S6 kinase (p70(s6k)) has a set of Ser and Thr residues (Ser411, Ser418, Ser424, and Thr421) phosphorylated in vivo by an unidentified kinase(s). These Ser/Thr sites are immediately followed by proline, a motif that is commonly seen in the substrates of cyclin-dependent kinases (Cdk) and mitogen-activated protein kinases. A previous study has shown that Cdc2 (Cdk1) indeed phosphorylates these p70(s6k) Ser/Thr residues in vitro. Here, we demonstrate that Cdc2-cyclin B… CONTINUE READING