Recently more and more evidences raise the possibility for the taste system in the role of the perception of lipids in mammals, and the fatty acid receptor CD36 has been proved to be as an important candidate receptor of fat taste. Fish has different taste modality with mammals. No information was known about the function of cd36 in fish taste till now. Here, using in situ hybridization and immunofluorescence technologies, we showed that fish cd36/Cd36 localized in taste buds. Real-time PCR technology demonstrated that, in zebrafish cd36 (zcd36)-transfected cells, linoleic acid (LA) increased the expression level of tryptophan hydroxylase-1 (TPH-1), which encodes the enzyme involved in the biosynthesis of monoamine neurotransmitter of 5-HT. Moreover, the LA-induced up-regulation expression of TPH-1 was significantly curtailed by SSO, a specific inhibitor of LCFA binding to CD36, suggesting zCd36 is implicated in the LA-induced release of neurotransmitter. Importantly, we observed that zcd36 gene knockout zebrafish reduced the preference for LA contrast to wild-type zebrafish. Together, our findings indicate that Cd36 is a candidate lipid sensor involved in the sensory detection of fatty acid in zebrafish.