Cavity hydration as a gateway to unfolding: an NMR study of hen lysozyme at high pressure and low temperature.


We have used low temperatures (down to -20°C) and high pressures (up to 2000 bar) to populate low-lying excited state conformers of hen lysozyme, and have analyzed their structures site-specifically using (15)N/(1)H two-dimensional HSQC NMR spectroscopy. The resonances of a number of residues were found to be selectively broadened, as the temperature was… (More)
DOI: 10.1016/j.bpc.2011.01.009


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