Cautionary note for DMPO spin trapping in the presence of iron ion.

@article{Makino1990CautionaryNF,
  title={Cautionary note for DMPO spin trapping in the presence of iron ion.},
  author={Keisuke Makino and Teruki Hagiwara and Akifumi Hagi and Makoto Nishi and Akiko Murakami},
  journal={Biochemical and biophysical research communications},
  year={1990},
  volume={172 3},
  pages={1073-80}
}
2-Hydroxy-5,5-dimethyl-1-pyrrolidinyloxy (DMPO-OH), which is known to be produced by spin trapping of hydroxyl radicals (.OH) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and has been a good monitor for detecting .OH in biological systems, has been examined by EPR for its production scheme in the presence of iron ion. In an aqueous DMPO solution containing ferric ion (Fe3+), DMPO-OH was produced and addition of methanol, a good scavenger for .OH, to this solution led to an aminoxyl radical… CONTINUE READING