Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome

@inproceedings{Marine2014CaughtIT,
  title={Caught in the middle with multiple displacement amplification: the myth of pooling for avoiding multiple displacement amplification bias in a metagenome},
  author={Rachel L Marine and Coleen McCarren and Vansay Vorrasane and Dan Nasko and Erin L. Crowgey and Shawn W. Polson and K. Eric Wommack},
  booktitle={Microbiome},
  year={2014}
}
BACKGROUND Shotgun metagenomics has become an important tool for investigating the ecology of microorganisms. Underlying these investigations is the assumption that metagenome sequence data accurately estimates the census of microbial populations. Multiple displacement amplification (MDA) of microbial community DNA is often used in cases where it is difficult to obtain enough DNA for sequencing; however, MDA can result in amplification biases that may impact subsequent estimates of population… CONTINUE READING
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