Cationic residues 53 and 56 control the anion-induced interfacial k*cat activation of pancreatic phospholipase A2.


Added NaCl or anionic amphiphiles increase the rate of hydrolysis of dispersions of zwitterionic phospholipid by pancreatic phospholipase A2 (PLA2). Two effects of the negative charge at the interface have been dissected: enhanced binding of the enzyme to the interface, and k*cat activation of the enzyme at the interface [Berg et al. (1997) Biochemistry 36… (More)


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