Cationic polyaspartamide-based nanocomplexes mediate siRNA entry and down-regulation of the pro-inflammatory mediator high mobility group box 1 in airway epithelial cells.

@article{Gioia2015CationicPN,
  title={Cationic polyaspartamide-based nanocomplexes mediate siRNA entry and down-regulation of the pro-inflammatory mediator high mobility group box 1 in airway epithelial cells.},
  author={Sante Di Gioia and Carla Sardo and Giuliana Belgiovine and Daniela Triolo and M D'apolito and Stefano Castellani and Annalucia Carbone and Ida Giardino and Gaetano Giammona and Gennara Cavallaro and Massimo Conese},
  journal={International journal of pharmaceutics},
  year={2015},
  volume={491 1-2},
  pages={359-66}
}
High-mobility group box 1 (HMGB1) is a nonhistone protein secreted by airway epithelial cells in hyperinflammatory diseases such as asthma. In order to down-regulate HMGB1 expression in airway epithelial cells, siRNA directed against HMGB1 was delivered through nanocomplexes based on a cationic copolymer of poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) by using H441 cells. Two copolymers were used in these experiments bearing respectively spermine side chains (PHEA-Spm) and both spermine and… CONTINUE READING