Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins.

Abstract

We examine the influence of cationic poly(amidoamine) (PAMAM) dendrimers on capillary electroseparation-UV analysis of proteins. PAMAMs adsorbing to the capillary surface suppressed the wall-adsorption of proteins; meanwhile, PAMAMs added to the buffer exhibited selectivity toward proteins. Presence of 3 x 10⁻⁴  g/mL PAMAM generation one (G 1.0) in 30 mM phosphate, at pH 2.6, rendered significant enhancement in separation efficiency; the merged peaks of myoglobin and trypsin inhibitor were separated. Moreover, the protein-dendrimer interactions changed the inherent UV absorbance profiles of proteins. UV-Vis study showed that the absorbance of cytochrome C and transferrin increased at the detection wavelength of 214 nm; their detection sensitivity enhanced by 2.44 and 2.01-folds, respectively, with addition of 5 x 10⁻⁴  g/mL PAMAM G 1.0.

DOI: 10.1002/elps.201000440

Cite this paper

@article{Liu2011CationicPD, title={Cationic poly(amidoamine) dendrimers as additives for capillary electroseparation and detection of proteins.}, author={Qingchun Liu and Jing Tian and Chenling Zhang and Huan Yang and Yang Liu and Weidong Qin and Zhengping Liu}, journal={Electrophoresis}, year={2011}, volume={32 11}, pages={1302-8} }