Cationic lipid and polymer-based gene delivery to human pancreatic islets.

@article{Mahato2003CationicLA,
  title={Cationic lipid and polymer-based gene delivery to human pancreatic islets.},
  author={Ram I Mahato and James Henry and Ajit S. Narang and Omaima M Sabek and Daniel W Fraga and Malak Y Kotb and A Osama Gaber},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2003},
  volume={7 1},
  pages={89-100}
}
Transplantation of pancreatic islets has great potential for treating Type I diabetes. Ex vivo gene therapy may promote re-vascularization or inhibit apoptosis of the islets and promote graft. In this study, we investigated the feasibility of non-viral gene delivery using Enhanced Green Fluorescent Protein (EGFP) and human Vascular Endothelial Growth Factor (hVEGF(165)) expression plasmids as model reporter and therapeutic genes. LipofectAMINE/pDNA and Superfect/pDNA complexes showed high… CONTINUE READING