Cathepsins D, B, and L in malignant human lung tissue.

  title={Cathepsins D, B, and L in malignant human lung tissue.},
  author={Peter Ledakis and W T Tester and Nurit Rosenberg and D Romero-Fischmann and Ierachmiel Daskal and Tamara T Lah},
  journal={Clinical cancer research : an official journal of the American Association for Cancer Research},
  volume={2 3},
The levels of cathepsins in malignant and surrounding nonmalignant lung tissue were determined in 17 non-small cell lung cancer specimens. Cathepsin (Cat) D activity was assayed using hemoglobin, whereas Cat B and Cat L activities were assayed using fluorimetric substrates, benzoylcarbonyl-Ala-Arg-Arg-7-amino-4-methylcoumarine and benzoylcarbonyl-Phe-Arg-7-amino-4-methylcoumarine, respectively. Cat protein concentrations were determined using ELISAs. In malignant tissues, the activities of Cat… CONTINUE READING