Cathepsin L Plays an Important Role in the Lysosomal Degradation of L-Lactate Dehydrogenase

@article{Ohshita2006CathepsinLP,
  title={Cathepsin L Plays an Important Role in the Lysosomal Degradation of L-Lactate Dehydrogenase},
  author={Takeyuki Ohshita and Yuzo Hiroi},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2006},
  volume={70},
  pages={2254 - 2261}
}
  • T. OhshitaY. Hiroi
  • Published 23 September 2006
  • Biology, Chemistry
  • Bioscience, Biotechnology, and Biochemistry
A cystatin α-sensitive cysteine proteinase that plays an important role in the lysosomal inactivation and degradation of L-lactate dehydrogenase (LDH) was purified by column chromatography from an ammonium sulfate precipitate of lysosome extract prepared from rat livers. It was eluted with marked delay from cathepsins B and H in a Sephacryl S-200 column by its specific interaction with the gel, and then effectively separated from cathepsins B and H and other proteins. It was eluted with 0.5 M… 

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  • T. Ohshita
  • Biology, Chemistry
    Bioscience, biotechnology, and biochemistry
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