Cathepsin K in adipocyte differentiation and its potential role in the pathogenesis of obesity.

  • Yin Xiao, Han Junfeng, +9 authors Luo Min
  • Published 2006 in
    The Journal of clinical endocrinology and…

Abstract

CONTEXT The alteration of protein expression in white adipose tissue (WAT) may contribute to the pathogenesis of obesity. OBJECTIVE The aim of the present study was to uncover proteins differentially expressed in the WAT of overweight/obese subjects and study the role of the identified proteins in adipocyte differentiation. DESIGN AND SETTING Two-dimensional electrophoresis and matrix-assisted laser desorption ionization-time of flight-mass spectrometry were used to identify proteins differentially expressed in WAT between obese/overweight and control groups. Cathepsin K (CTSK), one of the proteins identified by the above methods, was highlighted to assess its effects on adipocyte differentiation through 3T3-L1 cell line. RESULTS Human visceral adipose tissue of overweight/obese subjects displayed a differential protein expression profile, compared with that of normal-weight controls. CTSK was up-regulated in the WAT of overweight/obese subjects, and it had a significant positive correlation with body mass index. In vitro study showed that CTSK expression and its enzyme activity gradually increased in the process of adipocyte differentiation. Moreover, E-64, an inhibitor of CTSK, could prevent adipocyte differentiation in a dose-dependent manner, which was characterized by the absence of triglyceride accumulation and glycerol contents. CONCLUSIONS CTSK, a cysteine protease involved in extracellular matrix remodeling, could be one of the determinants of adipocyte differentiation. CTSK may be involved in the pathogenesis of obesity by promoting adipocyte differentiation.

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@article{Xiao2006CathepsinKI, title={Cathepsin K in adipocyte differentiation and its potential role in the pathogenesis of obesity.}, author={Yin Xiao and Han Junfeng and Luo Tianhong and Wang Lu and Chen Shulin and Zhao Yu and Li Xiaohua and Jian Weixia and Zheng Sheng and Gu Yanyun and Li Guo and Luo Min}, journal={The Journal of clinical endocrinology and metabolism}, year={2006}, volume={91 11}, pages={4520-7} }