Cathepsin B fraction active at physiological pH of 7.5 is of prognostic significance in squamous cell carcinoma of human lung.

@article{Werle1997CathepsinBF,
  title={Cathepsin B fraction active at physiological pH of 7.5 is of prognostic significance in squamous cell carcinoma of human lung.},
  author={Bernd Werle and B. J{\~A}¼lke and Tamara Lah and Eberhard Spiess and Werner Ebert},
  journal={British Journal of Cancer},
  year={1997},
  volume={75},
  pages={1137 - 1143}
}
In this study we examined both the pH dependence of cathepsin B (cath B) activity and its stability at physiological pH of 7.5 in lung tumours and normal lung tissue by means of fluorogenic assays with Z-Arg-Arg-AMC as specific substrate. Specificity was verified with the cath B blocking inhibitors E-64 and CA-074. With respect to pH dependence of activity, we found a deviation from a normal-shaped pH- activity curve. Besides the typical activity peak at pH 6.0, there were shoulders at pH 4.5-5… CONTINUE READING

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