Catching prompt metabolite dynamics in Escherichia coli with the BioScope at oxygen rich conditions.

@article{Mey2010CatchingPM,
  title={Catching prompt metabolite dynamics in Escherichia coli with the BioScope at oxygen rich conditions.},
  author={M. De Mey and Hilal Taymaz-Nikerel and Gino Baart and Hendrik Waegeman and J. Maertens and J. Heijnen and Walter M. van Gulik},
  journal={Metabolic engineering},
  year={2010},
  volume={12 5},
  pages={
          477-87
        }
}
The design and application of a BioScope, a mini plug-flow reactor for carrying out pulse response experiments, specifically designed for Escherichia coli is presented. Main differences with the previous design are an increased volume-specific membrane surface for oxygen transfer and significantly decreased sampling intervals. The characteristics of the new device (pressure drop, residence time distribution, plug-flow behavior and O2 mass transfer) were determined and evaluated. Subsequently, 2… Expand
42 Citations
Escherichia coli metabolism under short-term repetitive substrate dynamics: adaptation and trade-offs
  • 2
Comparison of the transient responses of Escherichia coli to a glucose pulse of various intensities
  • 25
Strategies for manipulation of oxygen utilization by the electron transfer chain in microbes for metabolic engineering purposes
  • G. Bennett, K. San
  • Medicine, Biology
  • Journal of Industrial Microbiology & Biotechnology
  • 2016
  • 6
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 35 REFERENCES
In vivo kinetics with rapid perturbation experiments in Saccharomyces cerevisiae using a second-generation BioScope.
  • 85
  • Highly Influential
Generating short-term kinetic responses of primary metabolism of Penicillium chrysogenum through glucose perturbation in the bioscope mini reactor.
  • 52
  • Highly Influential
Rapid sampling for analysis of in vivo kinetics using the BioScope: a system for continuous-pulse experiments.
  • 112
  • Highly Influential
Metabolome dynamic responses of Saccharomyces cerevisiae to simultaneous rapid perturbations in external electron acceptor and electron donor.
  • 38
  • Highly Influential
  • PDF
In vivo dynamics of glycolysis in Escherichia coli shows need for growth‐rate dependent metabolome analysis
  • 51
  • Highly Influential
Improved rapid sampling for in vivo kinetics of intracellular metabolites in Saccharomyces cerevisiae.
  • 201
Automated sampling device for monitoring intracellular metabolite dynamics.
  • 175
  • Highly Influential
In vivo analysis of glucose-induced fast changes in yeast adenine nucleotide pool applying a rapid sampling technique.
  • 198
...
1
2
3
4
...