Catching prompt metabolite dynamics in Escherichia coli with the BioScope at oxygen rich conditions.

@article{Mey2010CatchingPM,
  title={Catching prompt metabolite dynamics in Escherichia coli with the BioScope at oxygen rich conditions.},
  author={Marjan De Mey and Hilal Taymaz-Nikerel and Gino Baart and Hendrik Waegeman and J. Th. Maertens and Joseph J. Heijnen and Walter M. van Gulik},
  journal={Metabolic engineering},
  year={2010},
  volume={12 5},
  pages={
          477-87
        }
}
The design and application of a BioScope, a mini plug-flow reactor for carrying out pulse response experiments, specifically designed for Escherichia coli is presented. Main differences with the previous design are an increased volume-specific membrane surface for oxygen transfer and significantly decreased sampling intervals. The characteristics of the new device (pressure drop, residence time distribution, plug-flow behavior and O2 mass transfer) were determined and evaluated. Subsequently, 2… CONTINUE READING

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