Catalytic roles of CYP2C9 and its variants (CYP2C9*2 and CYP2C9*3) in lornoxicam 5'-hydroxylation.

@article{Iida2004CatalyticRO,
  title={Catalytic roles of CYP2C9 and its variants (CYP2C9*2 and CYP2C9*3) in lornoxicam 5'-hydroxylation.},
  author={Izumi Iida and Atsunori Miyata and Masayuki Arai and Mitsuyo Hirota and Masayuki Akimoto and Shohei Higuchi and Kaoru Kobayashi and Kan Chiba},
  journal={Drug metabolism and disposition: the biological fate of chemicals},
  year={2004},
  volume={32 1},
  pages={7-9}
}
The effects of allelic variants of CYP2C9 (CYP2C9*2 and CYP2C9*3) on lornoxicam 5'-hydroxylation were studied using the corresponding variant protein expressed in baculovirus-infected insect cells and human liver microsomes of known genotypes of CYP2C9. The results of the baculovirus expression system showed that CYP2C9.3 gives higher K(m) and lower V(max) values for lornoxicam 5'-hydroxylation than does CYP2C9.1. In contrast, K(m) and V(max) values of CYP2C9.1 and CYP2C9.2 for the reaction… CONTINUE READING