Catalytic mechanism of P-glycoprotein.

  title={Catalytic mechanism of P-glycoprotein.},
  author={Alan E. Senior},
  journal={Acta physiologica Scandinavica. Supplementum},
  • Alan E. Senior
  • Published 1998 in Acta physiologica Scandinavica. Supplementum
We generated Chinese hamster ovary cells which are highly multidrug-resistant by selection in colchicine. Purified plasma membranes from these cells are enriched in P-glycoprotein (Pgp), up to 32% w/w of membrane protein. From plasma membranes we purified Pgp to homogeneity and reconstituted it in proteoliposomes. Both plasma membranes and purified reconstituted Pgp show drug-stimulated ATPase activity (approximately 20 s-1), comparable to other transport ATPases. These materials enable… CONTINUE READING


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