Catalytic effects of elongation factor Ts on polypeptide synthesis.

  title={Catalytic effects of elongation factor Ts on polypeptide synthesis.},
  author={Tarmo Ruusala and M{\aa}ns Ehrenberg and Charles G. Kurland},
  journal={The EMBO journal},
  volume={1 1},
The kinetic parameters which characterize the interaction between elongation factor Tu (EF-Tu) and elongation factor Ts (EF-Ts) have been determined in a poly(uridylic acid)-primed translation system. The EF-Ts catalyzed release of GDP from EF-Tu was measured independently in a nucleotide exchange assay. We conclude that the rate-limiting step for the EF-Tu cycle in protein synthesis in the absence of EF-Ts is the release of GDP. By adding EF-Ts the time of this step is reduced from 90 s to 30… CONTINUE READING

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