Catalytic diversity and homotropic allostery of two Cytochrome P450 monooxygenase like proteins from Trichoderma brevicompactum

  title={Catalytic diversity and homotropic allostery of two Cytochrome P450 monooxygenase like proteins from Trichoderma brevicompactum},
  author={Razak Hussain and Indu Kumari and Shikha Sharma and Mushtaq Ahmed and Tabreiz A. Khan and Yusuf Akhter},
  journal={JBIC Journal of Biological Inorganic Chemistry},
Trichothecenes are the secondary metabolites produced by Trichoderma spp. Some of these molecules have been reported for their ability to stimulate plant growth by suppressing plant diseases and hence enabling Trichoderma spp. to be efficiently used as biocontrol agents in modern agriculture. Many of the proteins involved in the trichothecenes biosynthetic pathway in Trichoderma spp. are encoded by the genes present in the tri cluster. Tri4 protein catalyzes three consecutive oxygenation… 
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Fungal P450 monooxygenases - the diversity in catalysis and their promising roles in biocontrol activity
This mini review will provide updated information on different fungal P450 monooxygenases, their genetic diversity, and their role in catalyzing various biochemical reactions leading to the production of plant growth promoting secondary metabolites.
Interplay between two spin states determines the hydroxylation catalyzed by P450 monooxygenase from Trichoderma brevicompactum
The density functional theory (DFT)‐quantum mechanics (QM) approach is applied to elucidate the hydroxylation of EPT by using a model active species of P450 (Cpd I) by finding out transition‐state structure, intermediate, and product complexes for the two spin states at different potential energy surfaces.
Formation and Cleavage of C-C Bonds by Enzymatic Oxidation-Reduction Reactions.
Many oxidation-reduction (redox) enzymes, particularly oxygenases, have roles in reactions that make and break C-C bonds, and many of the enzymes involve metals, but of these, iron is clearly predominant.


Involvement of Trichoderma Trichothecenes in the Biocontrol Activity and Induction of Plant Defense-Related Genes
The results indicate that the tri4 gene disruption resulted in a reduced antifungal activity against Botrytis cinerea and Rhizoctonia solani and also in a reduce ability to induce the expression of tomato plant defense-related genes belonging to the salicylic acid (SA) and jasmonate (JA) pathways against B. cine Andrea.
Identification of Loci and Functional Characterization of Trichothecene Biosynthesis Genes in Filamentous Fungi of the Genus Trichoderma
Although some characteristics of the tri/TRI cluster have been conserved during evolution of Trichoderma and Fusarium, the cluster has undergone marked changes, including gene loss and/or gain, gene rearrangement, and divergence of gene function.
Molecular and Genetic Studies of Fusarium Trichothecene Biosynthesis: Pathways, Genes, and Evolution
The current understanding of the pathways of biosynthesis, the functions of cloned Tri genes, and the evolution of Tri genes are summarized, focusing on Fusarium species.
Mechanism of inhibition of eukaryotic protein synthesis by trichothecene fungal toxins.
It is concluded that nivalenol, T-2 toxin, and verrucarin A are potent and highly selective inhibitors of polypeptide chain initiation in eukaryotes, whereas trichodermin inhibits chain elongation and (or) termination.
Cloning and Functional Analysis of tri14 in Trichoderma brevicompactum
This study isolated the full cDNA and DNA sequence of Tbtri14 from T. brevicompactum 0248 and found it encodes 364 amino acids with a 59 bp intron, implying that TBTri14 may not be directly involved in trichodermin biosynthesis, Instead, it may act as a gene regulator, such as a repressor for Tbti11.
Deciphering the protein translation inhibition and coping mechanism of trichothecene toxin in resistant fungi.
The interactions of trichodermin with petidyltransferase centre of ribosomal complex was studied by molecular dynamics and in silico interaction methods to demonstrate its mechanism of action and to decipher the possible reason how it may inhibit protein synthesis at the ribosome complex.
Fungal Cytochrome P450 Monooxygenases: Their Distribution, Structure, Functions, Family Expansion, and Evolutionary Origin
The comparison of fungal CYPomes suggests that generally fungi possess abundant CYPs belonging to a variety of families with the two global families CYP51 and CYP61, indicating individuation of CYPome during the evolution of fungi.
Diversity of P450 enzymes in the biosynthesis of natural products.
Engineering microbial-derived P450 enzymes to accommodate alternative substrates and add new functions continues to be an important near- and long-term practical goal driving the structural characterization of these molecules.