Catalysis of amide proton exchange by the molecular chaperones GroEL and SecB.

@article{Zahn1996CatalysisOA,
  title={Catalysis of amide proton exchange by the molecular chaperones GroEL and SecB.},
  author={Ralph Zahn and Sarah Perrett and G Stenberg and A. R. Fersht},
  journal={Science},
  year={1996},
  volume={271 5249},
  pages={
          642-5
        }
}
Hydrogen-deuterium exchange of 39 amide protons of Bacillus amyloliquefaciens ribonuclease (barnase) was analyzed by two-dimensional nuclear magnetic resonance in the presence of micromolar concentrations of the molecular chaperones GroEL and SecB. Both chaperones bound to native barnase under physiological conditions and catalyzed exchange of deeply buried amide protons with solvent. Such exchange required complete unfolding of barnase, which occurred in the complex with the chaperones… CONTINUE READING

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