Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.

@article{Shukla2009CatalaseAS,
  title={Catalase and superoxide dismutase double staining zymogram technique for Deinococcus and Kocuria species exposed to multiple stresses.},
  author={Manish Shukla and Radhika S Yadav and Anjana Desai},
  journal={Journal of basic microbiology},
  year={2009},
  volume={49 6},
  pages={
          593-7
        }
}
Superoxide dismutase (SOD) and catalase expression is associated with oxidative stress. Existing techniques for the individual staining of SOD and catalase have been described in the past. The objective of this study was to achieve a simple and rapid technique for the double staining of bacterial SOD and catalase on the same polyacrylamide gel. SOD detection was carried out using nitro-blue tetrazolium (NBT) dye reduction followed by ferricyanide precipitation for negative staining of the… CONTINUE READING

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