Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame

@article{Jacob1991CataboliteRO,
  title={Catabolite repression of the operon for xylose utilization from Bacillus subtilis W23 is mediated at the level of transcription and depends on a cis site in the xylA reading frame},
  author={Sabine Jacob and Rudolf Allmansberger and Dagmar G{\"a}rtner and Wolfgang Hillen},
  journal={Molecular and General Genetics MGG},
  year={1991},
  volume={229},
  pages={189-196}
}
The Bacillus subtilis xyl operon encoding enzymes for xylose utilization is repressed in the absence of xylose and in the presence of glucose. Transcriptional fusions of spoVG-lacZ to this operon show regulation of β-galactosidase expression by glucose, indicating that glucose repression operates at the level of transcription. A similar result is obtained when glucose is replaced by glycerol, thus defining a general catabolite repression mechanism. A deletion of xylR, which encodes the xylose… CONTINUE READING
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