Catabolite-mediated mutations in alternate toluene degradative pathways in Pseudomonas putida.

@article{Leddy1995CatabolitemediatedMI,
  title={Catabolite-mediated mutations in alternate toluene degradative pathways in Pseudomonas putida.},
  author={Menu B Leddy and D W Phipps and Harry F. Ridgway},
  journal={Journal of bacteriology},
  year={1995},
  volume={177 16},
  pages={4713-20}
}
Pseudomonas putida 54g grew on mineral salts with toluene and exhibited catechol-2,3-dioxygenase (C23O) activity, indicating a meta pathway. After 10 to 15 days on toluene, nondegrading (Tol-) variants approached nearly 10% of total CFU. Auxotrophs were not detected among variants, suggesting selective loss of catabolic function(s). Variant formation was substrate dependent, since Tol- cells were observed on neither ethylbenzene, glucose, nor peptone-based media nor when toluene catabolism was… CONTINUE READING
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