Catabolism of protocatechuate by Bacillus macerans.

  title={Catabolism of protocatechuate by Bacillus macerans.},
  author={Ronald L. Crawford and J W Bromley and P E Perkins-Olson},
  journal={Applied and environmental microbiology},
  volume={37 3},
An aerobic endospore-forming bacterium, tentatively identified as a strain (JJ-lb) of Bacillus macerans, was isolated by enrichment on 4-hydroxybenzoate (4HBA), using as an inoculum soil taken from a 50 degrees C Iadho hot spring. Enzymatic analyses of cells grown on succinate and 4HBA indicated that strain JJ-1b degrades 4HBA by way of the novel protocatechuate (PCA) 2,3-dioxygenase pathway. Purification of the PCA 2,3-dioxygenase by affinity chromatography allowed the first observation of the… CONTINUE READING

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