Cassette labeling for facile construction of energy transfer fluorescent primers.

@article{Ju1996CassetteLF,
  title={Cassette labeling for facile construction of energy transfer fluorescent primers.},
  author={Jingyue Ju and Alexander N. Glazer and Richard A Mathies},
  journal={Nucleic acids research},
  year={1996},
  volume={24 6},
  pages={1144-8}
}
DNA primer sets, labeled with two fluorescent dyes to exploit fluorescence energy transfer (ET), can be efficiently excited with a single laser line and emit strong fluorescence at distinctive wavelengths. Such ET primers are superior to single fluorophore-labeled primers for DNA sequencing and other multiple color-based analyses [J. Ju, C. Ruan, C. W. Fuller, A. N. Glazer and R. A. Mathies (1995) Proc. Natl. Acad. Sci. USA 92, 4347-4351]. We describe here a novel method of constructing… CONTINUE READING

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