Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.

@article{Brown2007CaspaseIB,
  title={Caspase inhibition blocks cell death and results in cell cycle arrest in cytokine-deprived hematopoietic cells.},
  author={Nicholas M Brown and Sean M. Martin and Nick Maurice and Tomomi Kuwana and Charles M. Knudson},
  journal={The Journal of biological chemistry},
  year={2007},
  volume={282 4},
  pages={2144-55}
}
Cytokine deprivation has been classically used to study molecular processes of apoptosis. Following interleukin (IL)-3 withdrawal in FL5.12 cells, Bax undergoes a conformational change that results in its mitochondria targeting, cytochrome c release, activation of caspase-9, and apoptosis. Cells overexpressing Casp9DN (dominant negative caspase-9) or treated with the caspase inhibitor Q-VD-OPh increased viability but failed to increase clonogenic survival. We find that caspase-inhibited cells… CONTINUE READING