Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana.

@article{Menze2007CaspaseAD,
  title={Caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, Artemia franciscana.},
  author={Michael A. Menze and Steven C. Hand},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  year={2007},
  volume={292 5},
  pages={
          R2039-47
        }
}
  • M. Menze, S. Hand
  • Published 1 May 2007
  • Biology
  • American journal of physiology. Regulatory, integrative and comparative physiology
Evaluation of apoptotic processes downstream of the mitochondrion reveals caspase-9- and low levels of caspase-3-like activities in partly purified extracts of Artemia franciscana embryos. However, in contrast to experiments with extracts of human hepatoma cells, cytochrome c fails to activate caspase-3 or -9 in extracts from A. franciscana. Furthermore, caspase-9 activity is sensitive to exogenous calcium. The addition of 5 mM calcium leads to a 4.86 +/- 0.19 fold (SD) (n = 3) increase in… 

Figures and Tables from this paper

Mitochondria in energy-limited states: mechanisms that blunt the signaling of cell death
TLDR
Embryos of the brine shrimp, Artemia franciscana, survive extended periods of anoxia and diapause, and evidence indicates that opening of the mitochondrial permeability transition pore and release of cytochrome c (cyt-c) do not occur, and caspase activation in this crustacean is not dependent on cyt-c.
Anhydrobiosis-Associated Nuclear DNA Damage and Repair in the Sleeping Chironomid: Linkage with Radioresistance
TLDR
One of the factors explaining the relationship between radioresistance and the ability to undergo anhydrobiosis in the sleeping chironomid could be an adaptation to desiccation-inflicted nuclear DNA damage.
Mechanisms of animal diapause: recent developments from nematodes, crustaceans, insects, and fish.
TLDR
Evaluating current advancements in understanding of the mechanisms responsible for the remarkable phenotype, as well as environmental cues that signal entry and termination of the state of diapause concludes that a single suite of mechanisms does not regulate diAPause across all species and developmental stages.
Toxicity testing of Metarhizium conidia and toxins against aquatic invertebrates
TLDR
Meterhizium conidia and extracts affected the survival of all the aquatic invertebrates with An.
Molecular domains in epithelial salt cellNaCl of crustacean salt gland (Artemia).
  • F. Conte
  • Biology
    International review of cell and molecular biology
  • 2008
Gene expression, metabolic regulation and stress tolerance during diapause
  • T. MacRae
  • Biology
    Cellular and Molecular Life Sciences
  • 2010
TLDR
The purpose of this review is to consider regulatory and mechanistic strategies that are potentially key to metabolic control and stress tolerance during diapause, while remembering that organisms undergoing diAPause are as diverse as the processes itself.
Pathway of ATP utilization and duplex rRNA unwinding by the DEAD-box helicase, DbpA
TLDR
This work measured ATP utilization and duplex RNA unwinding by DbpA, a non-processive bacterial DEAD-box RNA helicase specifically activated by the peptidyl transferase center of 23S rRNA.
...
...

References

SHOWING 1-10 OF 45 REFERENCES
The Apaf-1 apoptosome: a large caspase-activating complex.
Structure of the apoptotic protease-activating factor 1 bound to ADP
TLDR
The 2.2-Å crystal structure of an ADP-bound, WD40-deleted Apaf-1 is reported, which reveals the molecular mechanism by which Apf-1 exists in an inactive state before ATP binding, and drives conformational changes that are essential for the formation of the apoptosome and the activation of caspase-9.
A nucleotide binding site in caspase-9 regulates apoptosome activation.
TLDR
Activity assays show that ATP and dATP, but not ADP or AMP, bind to the processed Csp9 p35/p10 and this nucleotide binding site might play an important and previously unrecognized role in regulating proCsp9 activation.
Physiological Concentrations of K+ Inhibit Cytochrome c-dependent Formation of the Apoptosome*
TLDR
The hypothesis that the normal intracellular concentrations of K+ act to safeguard the cell against inappropriate formation of the apoptosome complex, caused by the inadvertent release of small amounts of cytochromec, is supported.
Mitochondrial permeability transition in the crustacean Artemia franciscana: absence of a calcium-regulated pore in the face of profound calcium storage.
TLDR
The absence of a regulated MPTP in A. franciscana mitochondria could contribute to the extreme hypoxia tolerance in this crustacean, and it is speculated that absence of theregulated MPTP may be a general feature of invertebrates.
Formation of apoptosome is initiated by cytochrome c-induced dATP hydrolysis and subsequent nucleotide exchange on Apaf-1.
TLDR
The reconstitution of this pathway with purified recombinant Apaf-1, procaspase-9, procspase-3, and cytochrome c from horse heart is reported here and shows that dATP hydrolysis and exchange on Apf-1 are two required steps for apoptosome formation.
The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells
TLDR
It is found that loss of function mutations in dc3 and dc4, do not affect caspase activation during Drosophila development and that ectopic expression of DC3 or DC4 in Drosophile cells does not induce caspases activation.
Regulatory features of protein synthesis in isolated mitochondria from Artemia embryos.
TLDR
Mitochondrial protein synthesis was acutely sensitive to external pH, with 80% inhibition observed by lowering pH from 7.5 to 6.8, suggesting acidification of pHi may serve as one intracellular signal contributing to a coordinated suppression of both cytoplasmic and mitochondrial protein synthesis during transitions from active to anoxia-induced quiescent states.
...
...