Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing

@article{Sebbagh2001Caspase3mediatedCO,
  title={Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing},
  author={M. Sebbagh and C. Renvoiz{\'e} and J. Hamelin and N. Riche and J. Bertoglio and J. Br{\'e}ard},
  journal={Nature Cell Biology},
  year={2001},
  volume={3},
  pages={346-352}
}
Increased phosphorylation of myosin light chain (MLC) is necessary for the dynamic membrane blebbing that is observed at the onset of apoptosis. Here we identify ROCK I, an effector of the small GTPase Rho, as a new substrate for caspases. ROCK I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. ROCK proteins are known to regulate MLC-phosphorylation, and apoptotic cells… Expand
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References

SHOWING 1-10 OF 46 REFERENCES
Apoptotic Membrane Blebbing Is Regulated by Myosin Light Chain Phosphorylation
  • 472
  • PDF
Activation of hPAK65 by caspase cleavage induces some of the morphological and biochemical changes of apoptosis.
  • 196
  • PDF
Lamin proteolysis facilitates nuclear events during apoptosis
  • 618
  • PDF
Granzyme Release and Caspase Activation in Activated Human T-Lymphocytes*
  • 139
  • PDF
...
1
2
3
4
5
...