Carrier-mediated transport of riboflavin in Ashbya gossypii

  title={Carrier-mediated transport of riboflavin in Ashbya gossypii},
  author={Charlotte F{\"o}rster and Jos{\'e} Luis Revuelta and R. Andreas Kraemer},
  journal={Applied Microbiology and Biotechnology},
The filamentous hemiascomycete Ashbya gossypii is used for industrial riboflavin production. We examined riboflavin uptake and excretion at the plasma membrane using riboflavin auxotrophic and overproducing mutants. The riboflavin uptake system had low activity [V max=20±4 nmol min–1 g–1 mycelial dry weight (dw)] and high affinity (K M=40±12 µM). Inhibitor studies with the analogs FMN and FAD revealed high specificity of the uptake system. Excretion of riboflavin was not the consequence of non… CONTINUE READING