Carotenoids are effective inhibitors of in vitro hemolysis of human erythrocytes, as determined by a practical and optimized cellular antioxidant assay.

  title={Carotenoids are effective inhibitors of in vitro hemolysis of human erythrocytes, as determined by a practical and optimized cellular antioxidant assay.},
  author={Renan Campos Chist{\'e} and Marisa Freitas and Adriana Zerlotti Mercadante and Eduarda Fernandes},
  journal={Journal of food science},
  volume={79 9},
β-Carotene, zeaxanthin, lutein, β-cryptoxanthin, and lycopene are liposoluble pigments widely distributed in vegetables and fruits and, after ingestion, these compounds are usually detected in human blood plasma. In this study, we evaluated their potential to inhibit hemolysis of human erythrocytes, as mediated by the toxicity of peroxyl radicals (ROO•). Thus, 2,2'-azobis (2-methylpropionamidine) dihydrochloride (AAPH) was used as ROO• generator and the hemolysis assay was carried out in… 

β-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro models.

  • D. RibeiroA. Sousa E. Fernandes
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2020

Recovery and identification bio-active peptides from protein isolate of Spirulina platensis and their in vitro effectiveness against oxidative stress-induced erythrocyte hemolysis.

In conclusion, this study provided insights into the potential antioxidant function of the synthesized peptides originated from the bio-active peptides of S. platensis, which would contribute to the development of natural antioxidant from new protein resources.

Chlorinated Flavonoids Modulate the Inflammatory Process in Human Blood

The results demonstrated that 6-chloro-3′,4′,5,7-tetrahydroxyflavone deserves scientific attention due to its ability to modulate the reactive species and cytokines/chemokine production.

Optimization and multiple in vitro activity potentials of carotenoids from marine Kocuria sp. RAM1

Marine pigmented bacteria are a promising natural source of carotenoids. Kocuria sp. RAM1 was isolated from the Red Sea Bohadschia graeffei collected from Marsa Alam, Egypt, and used for carotenoids

Multitarget protection of Pterospartum tridentatum phenolic-rich extracts against a wide range of free radical species, antidiabetic activity and effects on human colon carcinoma (Caco-2) cells.

The obtained results proved that P. tridentatum extracts are a rich source of bioactive compounds and possess great antioxidant and antidiabetic activities, ability to protect human erythrocytes against oxidative damage, and to interfere with Caco-2 cells growth and to protect these cells when subject to tert-butyl hydroperoxide oxidative stress conditions.



Protective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytes.

Mechanism of free radical-induced hemolysis of human erythrocytes: hemolysis by water-soluble radical initiator.

The results indicate that either lipid peroxidation or redistribution of oxidized band 3 is not attributed only by itself to the hemolysis, despite lipidPeroxidation, and this model explained well the Hemolysis curves.

The carotenoid beta-cryptoxanthin stimulates the repair of DNA oxidation damage in addition to acting as an antioxidant in human cells.

The biological properties of beta-cryptoxanthin, a common carotenoid, are investigated in cell culture model systems, using the comet assay to measure DNA damage and appear to depend on the enhancement of DNA repair as well as antioxidant protection against damage.

Antioxidant activities of astaxanthin and related carotenoids.

  • Y. Naguib
  • Chemistry
    Journal of agricultural and food chemistry
  • 2000
The antioxidant activities of astaxanthin and related carotenoids have been measured by employing a newly developed fluorometric assay based on 4,4-difluoro-3,5-bis(4-phenyl-1, 3-butadienyl)-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid, which showed the highest antioxidant activity toward peroxyl radicals.