• Corpus ID: 33631003

Carnitine transport in rat heart slices: I. The action of thiol reagents on the acetylcarnitine/carnitine exchange.

@article{Sartorelli1985CarnitineTI,
  title={Carnitine transport in rat heart slices: I. The action of thiol reagents on the acetylcarnitine/carnitine exchange.},
  author={Lodovico Sartorelli and M. Ciman and Noris Siliprandi},
  journal={The Italian journal of biochemistry},
  year={1985},
  volume={34 4},
  pages={
          275-81
        }
}
Rat heart slices show a permeability barrier that can be crossed by carnitine but not by sucrose and inulin. The integrity of thiol groups of heart cell membrane is essential for the uptake of carnitine. N-ethylmaleimide inhibits the transport into heart slices which is insensitive to Mersalyl. On the contrary both N-ethylmaleimide and Mersalyl inhibit acetyl carnitine/carnitine exchange. The amount of thiol groups titrated by the above reagents are related to the extent of exchange inhibition. 

Myocardial carnitine transport.

It is demonstrated that in heart slices carnitine-deoxycarnitine exchange, occurring in a close one to one ratio, is (i) insensitive to both glycolysis and oxidative phosphorylation inhibitors and (ii) sensitive to thiol reagents, such as NEM and Mersalyl.

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Transport of L-carnitine into skeletal muscle was investigated using rat sarcolemmal membrane vesicles and found the existence of more than one carnitine carrier in skeletal muscle suggests potential-dependent transport.

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Since ATP is not cell permeant, a decrease in its content cannot be restored by extracellular sources. Thus, in every cell ATP consumption has to be matched by comparable rates of ATP production. By

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  • E. Brass
  • Biology, Medicine
    The American journal of clinical nutrition
  • 2000
Clinical trials integrating physiologic, biochemical, and pharmacologic assessments are needed to definitively clarify any effects of carnitine on exercise performance in healthy persons.