Cargo binding promotes KDEL receptor clustering at the mammalian cell surface

@article{Becker2016CargoBP,
  title={Cargo binding promotes KDEL receptor clustering at the mammalian cell surface},
  author={Bj{\"o}rn Becker and M. Reza Shaebani and Domenik Rammo and Tobias Bubel and Ludger Santen and Manfred J. Schmitt},
  journal={Scientific Reports},
  year={2016},
  volume={6}
}
Transmembrane receptor clustering is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense receptor/ligand interactions and subsequently translate an exogenous signal into a cellular response. Despite that receptor cluster formation has been described for a wide variety of receptors, ranging from chemotactic receptors in bacteria to growth factor and neurotransmitter receptors in mammalian cells, a mechanistic understanding of the underlying molecular processes is still… 
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References

SHOWING 1-10 OF 52 REFERENCES
Anthrax toxin triggers endocytosis of its receptor via a lipid raft–mediated clathrin-dependent process
TLDR
It is found that although endocytosis of ATR is slow, clustering it into rafts either via PA heptamerization or using an antibody sandwich is necessary and sufficient to trigger efficient internalization and allow delivery of LF to the cytoplasm.
Minimal Mesoscale Model for Protein-Mediated Vesiculation in Clathrin-Dependent Endocytosis
TLDR
A minimal mesoscale model is presented which quantitatively explains several experimental observations on the process of vesicle nucleation induced by the clathrin-coated assembly prior to vesicles scission inClathrin dependent endocytosis.
Outside-in signaling – a brief review of GPCR signaling with a focus on the Drosophila GPCR family
TLDR
Findings that the Drosophila odorant and gustatory receptors are not bona fide GPCRs as is the case for their mammalian counterparts are discussed and a phylogenetic analysis of the bona fide Drosophile GPCR families is presented that suggest potential roles for several family members.
The Epidermal Growth Factor Receptor (EGFR) Promotes Uptake of Influenza A Viruses (IAV) into Host Cells
TLDR
First indications for an interplay of IAV with receptor tyrosine kinases (RTKs) with representative RTK family-members the epidermal growth factor receptor (EGFR) and the c-Met receptor were studied, showing that these receptors transmit entry relevant signals upon virus binding.
EphB/ephrinB Signaling in Cell Adhesion and Migration
TLDR
The EphBs and ephrinBs are specifically localized and modified to promote higher-order clustering and initiate of bidirectional signaling in cell adhesion and migration.
Endocytotic uptake and retrograde transport of a virally encoded killer toxin in yeast
We demonstrate that a virally encoded yeast ‘killer’ toxin is entering its eukaryotic target cell by endocytosis, subsequently travelling the yeast secretory pathway in reverse to exhibit its lethal
Mechanics of receptor-mediated endocytosis.
TLDR
It is shown that particles in the size range of tens to hundreds of nanometers can enter or exit cells via wrapping even in the absence of clathrin or caveolin coats, and an optimal particles size exists for the smallest wrapping time.
The network of receptors characterize B cell receptor micro- and macroclustering in a Monte Carlo model.
TLDR
An energy based Monte Carlo model is developed to investigate the mechanism of B-cell receptor clustering upon ligation with soluble antigens and shows that mutual attraction between nearest neighbor receptor pairs can lead to microclusters of B cell receptors, but it is not sufficient for receptor macroclustering.
...
...