Cargo binding and regulatory sites in the tail of fungal conventional kinesin

@article{Seiler2000CargoBA,
  title={Cargo binding and regulatory sites in the tail of fungal conventional kinesin},
  author={Stephan Seiler and Jochen Kirchner and Christian Horn and Athina Kallipolitou and Guenther Woehlke and Manfred Schliwa},
  journal={Nature Cell Biology},
  year={2000},
  volume={2},
  pages={333-338}
}
Here, using a quantitative in vivo assay, we map three regions in the carboxy terminus of conventional kinesin that are involved in cargo association, folding and regulation, respectively. Using C-terminal and internal deletions, point mutations, localization studies, and an engineered ‘minimal’ kinesin, we identify five heptads of a coiled-coil domain in the kinesin tail that are necessary and sufficient for cargo association. Mutational analysis and in vitro ATPase assays highlight a… CONTINUE READING

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