Cargo binding and regulatory sites in the tail of fungal conventional kinesin

  title={Cargo binding and regulatory sites in the tail of fungal conventional kinesin},
  author={Stephan Seiler and Jochen Kirchner and Christian Horn and Athina Kallipolitou and Guenther Woehlke and Manfred Schliwa},
  journal={Nature Cell Biology},
Here, using a quantitative in vivo assay, we map three regions in the carboxy terminus of conventional kinesin that are involved in cargo association, folding and regulation, respectively. Using C-terminal and internal deletions, point mutations, localization studies, and an engineered ‘minimal’ kinesin, we identify five heptads of a coiled-coil domain in the kinesin tail that are necessary and sufficient for cargo association. Mutational analysis and in vitro ATPase assays highlight a… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Explore Further: Topics Discussed in This Paper


Publications citing this paper.
Showing 1-10 of 69 extracted citations

Crosslinking studies on the conventional kinesin of Neurospora crassa

Katrin Hahlen Erstgutachter
View 6 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-10 of 55 references

Formation of the compact confomer of kinesin requires a COOH-terminal heavy chain domain and inhibits microubule-stimulated ATPase activity

Stock, F M.
J. Biol • 1999

activation of membrane-associated kinesin during development

J. D. Lane, Allan, V. J. Microtubule-based endoplasmic reticulum motilit laevis
Mol. Biol. Cell. 10, 1909–1922 • 1999

Similar Papers

Loading similar papers…