Cardiovascular imaging using two-photon microscopy.

@article{Scherschel2008CardiovascularIU,
  title={Cardiovascular imaging using two-photon microscopy.},
  author={John A. Scherschel and Michael Rubart},
  journal={Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada},
  year={2008},
  volume={14 6},
  pages={
          492-506
        }
}
Two-photon excitation microscopy has become the standard technique for high resolution deep tissue and intravital imaging. It provides intrinsic three-dimensional resolution in combination with increased penetration depth compared to single-photon confocal microscopy. This article will describe the basic physical principles of two-photon excitation and will review its multiple applications to cardiovascular imaging, including second harmonic generation and fluorescence laser scanning microscopy… CONTINUE READING

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