Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays

@inproceedings{Miwa2016CarboxylesteraseCA,
  title={Carboxylesterase converts Amplex red to resorufin: Implications for mitochondrial H2O2 release assays},
  author={Satomi Miwa and Achim Treumann and Amy Bell and Giulio Vistoli and Glyn Nelson and Sam Hay and Thomas von Zglinicki},
  booktitle={Free radical biology & medicine},
  year={2016}
}
Amplex Red is a fluorescent probe that is widely used to detect hydrogen peroxide (H2O2) in a reaction where it is oxidised to resorufin by horseradish peroxidase (HRP) as a catalyst. This assay is highly rated amongst other similar probes thanks to its superior sensitivity and stability. However, we report here that Amplex Red is readily converted to resorufin by a carboxylesterase without requiring H2O2, horseradish peroxidase or oxygen: this reaction is seen in various tissue samples such as… CONTINUE READING

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